Robust and automated three-dimensional segmentation of densely packed cell nuclei in different biological specimens with Lines-of-Sight decomposition.
Biena MathewAlexander SchmitzSilvia Muñoz-DescalzoNariman AnsariFrancesco PampaloniErnst H. K. StelzerSabine Christine FischerPublished in: BMC Bioinform. (2015)
Keyphrases
- cell nuclei
- automated analysis
- completely automated
- tissue samples
- three dimensional
- fluorescence microscopy images
- microscope images
- densely packed
- low contrast
- automated detection
- quantitative analysis
- cell nucleus
- microscopy images
- watershed transform
- quantitative evaluation
- histopathological images
- segmentation method
- level set
- medical images
- segmentation algorithm
- image segmentation
- visual analysis
- cancer diagnosis
- multi channel
- nonrigid registration
- computer vision
- line drawings
- shape prior
- region growing
- mathematical morphology
- medical imaging
- gray scale
- gray level
- edge detection
- image sequences
- image processing